Pharmacological effect of Wobenzym on the blood coagulation system

Korpan M.I., Korpan N.N., Tschekman I.S., Fialka V.

Pharmacology Dep., National Medical University, Kiev, Ukraine
Dep. of physiotherapy and rehabilitation, Vienna, Austria

Likarska Sprava 1997, No. 4, pp. 70-72 

Abstract in  Russian.

We investigated the effect of Wobenzym on blood clotting and fibrinolysis in the intact animals and animals with toxic hepatitis. Experiments were done with 90 Wistar rats, body weight 180-240 g.
Following parameters which characterize blood coagulation system and fibrinolysis were observed in plasma:

• time of thrombocytary plasma recalcification
• prothrombin time of thrombocyte-free plasma
• thrombin time of thrombocyte-free plasma
• fibrinolytic activity of blood

Wobenzym dragees were ground in a mortar, dissolved in saline and administered to the rats by gastric probe at the dose of 5, 20 and 100 mg/kg in 2 ml. Control animals received the same volume of saline. To provoke a toxic hepatitis, animals were subcutaneously administered with carbon tetrachloride solution (4 ml per kg) for 4 days.

Starting at the day 5 on, animals receiving CCl₄ were divided into 4 groups:

1. control
2. test group, receiving Wobenzym at the dose of 5 mg per 1 kg of rat body weight. The preparation was administered using a gastric probe
3. test group, receiving Wobenzym at the dose of 20 mg per 1 kg of rat body weight
4. test group, receiving Wobenzym at the dose of 100 mg per 1 kg of rat body weight

Wobenzym at doses of 5, 20, and 100 mg/kg showed no effect on blood coagulation and fibrinolysis after 60 min following intragastric administration.
CCl₄ provoked changes in blood coagulation - prothrombin time increased by 17.2 %, time of thrombocytary plasma recalcification decreased by 10.9 % and fibrinolysis time by 28.1 %. Thrombin time did not change. Wobenzym at doses of 5, 20 and 100 mg/kg showed normalizing effect on the above mentioned parameters in rats with toxic hepatitis.